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OTC Monoclonal antibody

OTC Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C7

Cat No : 67553-1-Ig

Print datasheet

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

验证数据展示

  • WB analysis using 67553-1-Ig

    WB analysis using 67553-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67553-1-Ig

    IHC staining of human liver cancer using 67553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OTC antibody included

经过测试的应用

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OTC Ready-To-Use IHC Kit. OTC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67553-1-Ig targets OTC in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen OTC fusion protein Ag24186 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ornithine carbamoyltransferase
Synonyms OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase
Calculated Molecular Weight 354 aa, 40 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC074745
Gene Symbol OTC
Gene ID (NCBI) 5009
RRIDAB_2882767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00480
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

实验方案

Product Specific Protocols
WB protocol for OTC antibody 67553-1-IgDownload protocol
IHC protocol for OTC antibody 67553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols