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验证数据展示

  • WB analysis using 21996-1-AP

    WB analysis using 21996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21996-1-AP (OSCAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

OSCAR Polyclonal antibody

OSCAR Polyclonal Antibody for WB, ELISA
Cat No. 21996-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Poly-Ig receptor 3, PIgR-3, PIgR3, Osteoclast-associated receptor, Osteoclast-associated immunoglobulin-like receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21996-1-AP targets OSCAR in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 OSCAR fusion protein Ag17366 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 osteoclast associated, immunoglobulin-like receptor
别名 Poly-Ig receptor 3, PIgR-3, PIgR3, Osteoclast-associated receptor, Osteoclast-associated immunoglobulin-like receptor
计算分子量 282 aa, 31 kDa
观测分子量40 kDa
GenBank蛋白编号BC035023
基因名称 OSCAR
Gene ID (NCBI) 126014
RRIDAB_3669383
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYS5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteoclast-associated receptor (OSCAR) is an FcRγ-associated receptor that belongs to the leukocyte receptor cluster (LRC). OSCAR is expressed by myeloid cells and is involved in antigen presentation and activation of human dendritic cells(PMID: 15155468; PMID: 15650060). OSCAR is expressed in osteoclast cells and involved in the differentiation of osteoclasts from peripheral blood mononuclear cells (PBMC). The N-glycosylated form of human OSCAR has a molecular weight of approximately 40-45 kDa, while 30 kDa corresponds to its fully non-glycosylated form (PMID: 15155468; 24902903; 27917924)

实验方案

Product Specific Protocols
WB protocol for OSCAR antibody 21996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols