验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21996-1-AP targets OSCAR in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag17366 Product name: Recombinant human OSCAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-282 aa of BC035023 Sequence: NVSLRCAGRLRNMSFVLYREGVAAPLQYRHSAQPWADFTLLGARAPGTYSCYYHTPSAPYVLSQRSEVLVISWEGEGPEARPASSAPGMQAPGPPPSDPGAQAPSLSSFRPRGLVLQPLLPQTQDSWDPAPPPSDPGV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | osteoclast associated, immunoglobulin-like receptor |
| 别名 | Poly-Ig receptor 3, PIgR-3, PIgR3, Osteoclast-associated receptor, Osteoclast-associated immunoglobulin-like receptor |
| 计算分子量 | 282 aa, 31 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC035023 |
| 基因名称 | OSCAR |
| Gene ID (NCBI) | 126014 |
| RRID | AB_3669383 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYS5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Osteoclast-associated receptor (OSCAR) is an FcRγ-associated receptor that belongs to the leukocyte receptor cluster (LRC). OSCAR is expressed by myeloid cells and is involved in antigen presentation and activation of human dendritic cells(PMID: 15155468; PMID: 15650060). OSCAR is expressed in osteoclast cells and involved in the differentiation of osteoclasts from peripheral blood mononuclear cells (PBMC). The N-glycosylated form of human OSCAR has a molecular weight of approximately 40-45 kDa, while 30 kDa corresponds to its fully non-glycosylated form (PMID: 15155468; 24902903; 27917924)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OSCAR antibody 21996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
