验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human testis tissue, mouse lung tissue |
| Positive IHC detected in | human testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
11879-1-AP targets OSBPL9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2476 Product name: Recombinant human OSBPL9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-558 aa of BC025978 Sequence: DLTKVVLPTFILERRSLLEMYADFFAHPDLFVSISDQKDPKDRMVQVVKWYLSAFHAGRKGSVAKKPYNPILGEIFQCHWTLPNDTEENTELVSEGPVPWVSKNSVTFVAEQVSHHPPISAFYAECFNKKIQFNAHIWTKSKFLGMSIGVHNIGQGCVSCLDYDEHYILTFPNGYGRSILTVPWVELGGECNINCSKTGYSANIIFHTKPFYGGKKHRITAEIFSPNDKKSFCSIEGEWNGVMYAKYATGENTVFVDTKKLPIIKKKVRKLEDQNEYESRSLWKDVTFNLKIRDIDAATEAKHRLEERQRAEARERKEKEIQWETRLFHEDGECWVYDEPLLKRLGAAKH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxysterol binding protein-like 9 |
| 别名 | ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9 |
| 计算分子量 | 736 aa, 83 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC025978 |
| 基因名称 | OSBPL9 |
| Gene ID (NCBI) | 114883 |
| RRID | AB_10638005 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SU4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ORP9 is expressed as a full-length (ORP9L) or truncated version missing the PH domain (ORP9S).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for OSBPL9 antibody 11879-1-AP | Download protocol |
| WB protocol for OSBPL9 antibody 11879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites | ||
J Cell Biol Phosphatidylserine regulates plasma membrane repair through tetraspanin-enriched macrodomains
| ||
