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OSBPL9 Polyclonal antibody

OSBPL9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11879-1-AP

Print datasheet

Synonyms

ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9

验证数据展示

  • WB analysis of human brain using 11879-1-AP

    WB analysis of human brain using 11879-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 11879-1-AP

    IHC staining of human testis using 11879-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 11879-1-AP

    IHC staining of human testis using 11879-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, human testis tissue, mouse lung tissue
Positive IHC detected inhuman testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11879-1-AP targets OSBPL9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen OSBPL9 fusion protein Ag2476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxysterol binding protein-like 9
Synonyms ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9
Calculated Molecular Weight 736 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC025978
Gene Symbol OSBPL9
Gene ID (NCBI) 114883
RRIDAB_10638005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ORP9 is expressed as a full-length (ORP9L) or truncated version missing the PH domain (ORP9S).

实验方案

Product Specific Protocols
WB protocol for OSBPL9 antibody 11879-1-APDownload protocol
IHC protocol for OSBPL9 antibody 11879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
View Article
humanWB

Cell Mol Life Sci

ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites

Authors - Ruyue He
View Article