OSBPL3 Polyclonal antibody

OSBPL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12417-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

OSBP3, OSBP related protein 3, ORP-3, ORP3, ORP 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 3 publications below

产品信息

12417-1-AP targets OSBPL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 OSBPL3 fusion protein Ag3044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oxysterol binding protein-like 3
别名 OSBP3, OSBP related protein 3, ORP-3, ORP3, ORP 3
计算分子量 887 aa, 101 kDa
观测分子量 95-110 kDa
GenBank蛋白编号BC017731
基因名称 OSBPL3
Gene ID (NCBI) 26031
RRIDAB_2156103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4L5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The oxysterol-binding protein-like (OSBPL) protein family includes nine members namely OSBPL2, OSBPL3, OSBPL5, OSBPL6, OSBPL7, OSBPL8, OSBPL9, OSBPL10, and OSBPL11. The OSBPL family proteins (OSBPLs) are located at the membrane to exchange molecules or signals between organelles and function as a necessary transporter. In addition to lipid transport, the OSBPLs are involved in the regulation of the actin cytoskeleton, cell polarity, and cell. Recently, the role of OSBPLs in cancer development has been gradually revealed. Upregulation of OSBPL3 could promote colorectal cancer. (PMID: 36918840)

实验方案

Product Specific Protocols
WB protocol for OSBPL3 antibody 12417-1-APDownload protocol
IHC protocol for OSBPL3 antibody 12417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

BMC Cancer

The expression, immune infiltration, prognosis, and experimental validation of OSBPL family genes in liver cancer

Authors - Kunpeng Tian
humanIHC

Biosci Rep

Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis.

Authors - Yu Zhang
humanIHC

Biosci Rep

Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis and clinical data from The Cancer Genome Atlas.

Authors - Yu Zhang
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