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OSBPL3 Polyclonal antibody

OSBPL3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12417-1-AP

Print datasheet

Synonyms

KIAA0704, ORP 3, ORP3, OSBP related protein 3, OSBP3, OSBPL3

验证数据展示

  • WB analysis using 12417-1-AP

    WB analysis using 12417-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12417-1-AP (OSBPL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12417-1-AP

    IHC staining of human colon cancer using 12417-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12417-1-AP

    IHC staining of human colon cancer using 12417-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 3 publications below

产品信息

12417-1-AP targets OSBPL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen OSBPL3 fusion protein Ag3044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxysterol binding protein-like 3
Synonyms KIAA0704, ORP 3, ORP3, OSBP related protein 3, OSBP3, OSBPL3
Calculated Molecular Weight 887 aa, 101 kDa
Observed Molecular Weight 95-110 kDa
GenBank Accession NumberBC017731
Gene Symbol OSBPL3
Gene ID (NCBI) 26031
RRIDAB_2156103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4L5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OSBPL3 antibody 12417-1-APDownload protocol
IHC protocol for OSBPL3 antibody 12417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

BMC Cancer

The expression, immune infiltration, prognosis, and experimental validation of OSBPL family genes in liver cancer

Authors - Kunpeng Tian
View Article
humanIHC

Biosci Rep

Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis.

Authors - Yu Zhang
View Article
humanIHC

Biosci Rep

Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis and clinical data from The Cancer Genome Atlas.

Authors - Yu Zhang
View Article