验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, K-562 cells, HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 3 publications below |
产品信息
12417-1-AP targets OSBPL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | OSBPL3 fusion protein Ag3044 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxysterol binding protein-like 3 |
| 别名 | OSBP3, OSBP related protein 3, ORP-3, ORP3, ORP 3 |
| 计算分子量 | 887 aa, 101 kDa |
| 观测分子量 | 95-110 kDa |
| GenBank蛋白编号 | BC017731 |
| 基因名称 | OSBPL3 |
| Gene ID (NCBI) | 26031 |
| RRID | AB_2156103 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H4L5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The oxysterol-binding protein-like (OSBPL) protein family includes nine members namely OSBPL2, OSBPL3, OSBPL5, OSBPL6, OSBPL7, OSBPL8, OSBPL9, OSBPL10, and OSBPL11. The OSBPL family proteins (OSBPLs) are located at the membrane to exchange molecules or signals between organelles and function as a necessary transporter. In addition to lipid transport, the OSBPLs are involved in the regulation of the actin cytoskeleton, cell polarity, and cell. Recently, the role of OSBPLs in cancer development has been gradually revealed. Upregulation of OSBPL3 could promote colorectal cancer. (PMID: 36918840)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OSBPL3 antibody 12417-1-AP | Download protocol |
| IHC protocol for OSBPL3 antibody 12417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cancer The expression, immune infiltration, prognosis, and experimental validation of OSBPL family genes in liver cancer | ||
Biosci Rep Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis. | ||
Biosci Rep Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis and clinical data from The Cancer Genome Atlas. |
