验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
文献引用应用 | WB, IF, ChIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | OSBPL2 fusion protein Ag6496 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | oxysterol binding protein-like 2 |
别名 | OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2 |
计算分子量 | 55 kDa |
观测分子量 | 51 kDa, 56 kDa |
GenBank蛋白编号 | BC000296 |
基因名称 | OSBPL2 |
Gene ID (NCBI) | 9885 |
RRID | AB_2156092 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H1P3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
IHC protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
IF protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
IP protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. | ||
JCI Insight Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling. | ||
Genet Med Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing. | ||
Mol Cell Endocrinol Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.
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Exp Cell Res OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.
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