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OSBPL2 Polyclonal antibody

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 14751-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, ELISA and More (2)

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 OSBPL2 fusion protein Ag6496 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oxysterol binding protein-like 2
别名 OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2
计算分子量 55 kDa
观测分子量 51 kDa, 56 kDa
GenBank蛋白编号BC000296
基因名称 OSBPL2
Gene ID (NCBI) 9885
RRIDAB_2156092
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1P3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OSBPL2 antibody 14751-1-APDownload protocol
IHC protocol for OSBPL2 antibody 14751-1-APDownload protocol
IF protocol for OSBPL2 antibody 14751-1-APDownload protocol
IP protocol for OSBPL2 antibody 14751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Protein Cell

ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover

Authors - Maomao Pu
humanWB,IF

Autophagy

OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.

Authors - Young Ik Koh
mouseIF

JCI Insight

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.

Authors - Hairong Shi
WB

Genet Med

Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.

Authors - Guangqian Xing
humanIF,ChIP

Mol Cell Endocrinol

Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.

Authors - Tamara Escajadillo
  • KD Validated
humanWB

Exp Cell Res

OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.

Authors - Cui Zhang
  • KO Validated
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