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验证数据展示

  • WB analysis using 11318-1-AP

    WB analysis using 11318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11318-1-AP (OSBPL11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11318-1-AP

    IHC staining of human ovary tumor using 11318-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11318-1-AP (OSBPL11 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with OSBPL11 antibody included
  • IP experiment of mouse testis using 11318-1-AP

    IP experiment of mouse testis using 11318-1-AP

    IP result of anti-OSBPL11 (IP:11318-1-AP, 4ug; Detection:11318-1-AP 1:300) with mouse testis tissue lysate 4000ug.

OSBPL11 Polyclonal antibody

OSBPL11 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11318-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OSBPL11 Ready-To-Use IHC Kit. OSBPL11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11318-1-AP targets OSBPL11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag1855

Product name: Recombinant human OSBPL11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-367 aa of BC010422

Sequence: MDLTRVVLPTFILEKRSLLEMYADFMSHPDLFIAITNGATAEDRMIRFVEYYLTSFHEGRKGAIAKKPYNPIIGETFHCSWKMPKSEVASSVFSSSSTQGVTNHAPLSGESLTQVGSDCYTVRFVAEQVSHHPPVSGFYAECTERKMCVNAHVWTKSKFLGMSIGVTMVGEGILSLLEHGEEYTFSLPCAYARSILTVPWVELGGKVSVNCAKTGYSASITFHTKPFYGGKLHRVTAEVKHNITNTVVCRVQGEWNSVLEFTYSNGETKYVDLTKLAVTKKRVRPLEKQDPFESRRLWKNVTDSLRESEIDKATEHKHTLEERQRTEERHRTETGTPWKTKYFIKEGDGWVYHKPLWKIIPTTQPAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oxysterol binding protein-like 11
别名 ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292
计算分子量 747 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC010422
基因名称 OSBPL11
Gene ID (NCBI) 114885
RRIDAB_2267735
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXB4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OSBPL11 antibody 11318-1-APDownload protocol
IHC protocol for OSBPL11 antibody 11318-1-APDownload protocol
IP protocol for OSBPL11 antibody 11318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
View Article