验证数据展示

WB analysis using 11096-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa cells using 11096-1-AP
IP result of anti-OSBP (11096-1-AP for IP and Detection).
IHC staining of human ovary tumor using 11096-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11096-1-AP (OSBP antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with OSBP antibody included
IF Staining of HeLa using 11096-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11096-1-AP (OSBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

OSBP Polyclonal antibody

OSBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11096-1-AP

产品说明书

引用文献(22)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Oxysterol-binding protein 1, Oxysterol binding protein 1, oxysterol binding protein, OSBP1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (22)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, rat kidney tissue, mouse kidney tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy OSBP Ready-To-Use IHC Kit. OSBP primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 19 publications below
IF	See 8 publications below
IP	See 1 publications below
CoIP	See 1 publications below

产品信息

11096-1-AP targets OSBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rabbit, yeast
免疫原	OSBP fusion protein Ag1578 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	oxysterol binding protein
别名	Oxysterol-binding protein 1, Oxysterol binding protein 1, oxysterol binding protein, OSBP1
计算分子量	89 kDa
观测分子量	89 kDa
GenBank蛋白编号	BC011581
基因名称	OSBP
Gene ID (NCBI)	5007
RRID	AB_2158220
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P22059
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for OSBP antibody 11096-1-AP	Download protocol
IHC protocol for OSBP antibody 11096-1-AP	Download protocol
IF protocol for OSBP antibody 11096-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
human	WB,IF	Gastroenterology Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking. Authors - Hongliang Wang View Article
human	WB	Cell Host Microbe The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry. Authors - Samad Amini-Bavil-Olyaee View Article
mouse	WB	Mol Metab Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc Transferase Authors - Samuel S Boyd View Article
human	WB,IF	Phytomedicine LW-213, a derivative of wogonin, triggers reticulophagy-mediated cell death in NSCLC via lysosomal damage combined with NPC1 inhibition Authors - Hongzheng Wang View Article
human	WB,IF	PLoS Pathog Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus. Authors - Cristina M Dorobantu KD Validated View Article

产品中心

应用中心

研究领域

cGMP 级蛋白

技术支持

促销活动

关于我们

抗体

免疫分析

PTG-Lab免疫学实验试剂

PTG-Cell细胞实验试剂

细胞因子&活性蛋白

重组蛋白

纳米抗体相关产品

空间多组学

表观遗传领域相关产品

研究领域

验证数据展示

WB analysis using 11096-1-AP

IP experiment of HeLa cells using 11096-1-AP

IHC staining of human ovary tumor using 11096-1-AP

IF Staining of HeLa using 11096-1-AP

OSBP Polyclonal antibody

OSBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.

The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.

Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc Transferase

LW-213, a derivative of wogonin, triggers reticulophagy-mediated cell death in NSCLC via lysosomal damage combined with NPC1 inhibition

Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus.