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验证数据展示

  • WB analysis using 82932-1-RR

    WB analysis using 82932-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 82932-1-RR

    IHC staining of mouse testis using 82932-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 82932-1-RR

    IHC staining of mouse testis using 82932-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 82932-1-RR

    FC experiment of HeLa using 82932-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of MCF-7 using 82932-1-RR

    WB analysis of MCF-7 using 82932-1-RR

    WB result of OS9 antibody (82932-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OS9 transfected MCF-7 cells.

  • Affinity and Kinetic Characterization of 82932-1-RR

    Affinity and Kinetic Characterization of 82932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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OS9 Recombinant monoclonal antibody

OS9 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82932-1-RR

产品说明书

CloneNo. 230149B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

230149B4, Amplified in osteosarcoma 9, Protein OS-9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82932-1-RR targets OS9 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0106

Product name: Recombinant human OS9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-435 aa of BC000532

Sequence: GRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 osteosarcoma amplified 9, endoplasmic reticulum associated protein
别名 230149B4, Amplified in osteosarcoma 9, Protein OS-9
计算分子量 76 kDa
观测分子量83-97 kDa
GenBank蛋白编号BC000532
基因名称 OS9
Gene ID (NCBI) 10956
RRIDAB_3670673
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13438
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

实验方案

Product Specific Protocols
WB protocol for OS9 antibody 82932-1-RRDownload protocol
IHC protocol for OS9 antibody 82932-1-RRDownload protocol
FC protocol for OS9 antibody 82932-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols