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ORM1/2 Monoclonal antibody

ORM1/2 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5F2H7

Cat No : 66097-1-Ig

Print datasheet

Synonyms

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

验证数据展示

  • WB analysis of human plasma using 66097-1-Ig

    WB analysis of human plasma using 66097-1-Ig

    0.8μL human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66097-1-Ig

    WB analysis using 66097-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • WB analysis using 66097-1-Ig

    WB analysis using 66097-1-Ig

    Untreated and PNGase F-treated lysates of HuH-7 cells were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human liver cancer using 66097-1-Ig

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66097-1-Ig

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66097-1-Ig

    IF Staining of HepG2 using 66097-1-Ig

    Immunofluorescent analysis of () fixed HepG2 cells using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:25.

  • FC experiment of HepG2 using 66097-1-Ig

    FC experiment of HepG2 using 66097-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ORM1/2 (66097-1-Ig, Clone:5F2H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman plasma tissue, HuH-7 cells, human testis tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 66097-1-Ig is ORM1/2 Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ORM1/2 fusion protein Ag19248 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name orosomucoid 1
Synonyms AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1
Calculated Molecular Weight 201 aa, 24 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC026238
Gene Symbol ORM1
Gene ID (NCBI) 5004
RRIDAB_2881496
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

实验方案

Product Specific Protocols
WB protocol for ORM1/2 antibody 66097-1-IgDownload protocol
IHC protocol for ORM1/2 antibody 66097-1-IgDownload protocol
IF protocol for ORM1/2 antibody 66097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioengineered

Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9.

Authors - Luo Qiong
View Article