验证数据展示
经过测试的应用
| Positive IP detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32706-1-AP targets ORAOV1 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38523 Product name: Recombinant human ORAOV1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of NM_153451.2 Sequence: MAGSQDIFDAIVMADERFHGEGYREGYEEGSSLGVMEGRQHGTLHGAKIGSEIGCYQGFAFAWKCLLHSCTTEKDSRKMKVLESLIGMIQKFPYDDPTYDKLHEDLDKIRGKFKQFCSLLNVQPDFKISAEGSGLSF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oral cancer overexpressed 1 |
| 别名 | CIAB1, LTO1, Oral cancer-overexpressed protein 1, Protein LTO1 homolog, TAOS1 |
| 计算分子量 | 15kDa，137aa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | NM_153451.2 |
| 基因名称 | ORAOV1 |
| Gene ID (NCBI) | 220064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WV07 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ORAOV1 (oral cancer overexpressed 1) is a candidate proto-oncogene that is frequently amplified and overexpressed in various human squamous cell carcinomas (SCCs), including oral, esophageal, and cervical cancers. In cervical cancer, silencing of ORAOV1 significantly inhibits the growth of HeLa cells by causing S-phase cell cycle arrest and activating apoptosis. Additionally, ORAOV1 is overexpressed in a variety of solid tumors and may promote tumor development by preventing ribosomal damage induced by reactive oxygen species (ROS). Therefore, ORAOV1 is not only an important tumor biomarker but also a potential therapeutic target for multiple cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ORAOV1 antibody 32706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
