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验证数据展示

  • WB analysis of A375 using 28637-1-AP

    WB analysis of A375 using 28637-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 28637-1-AP (ORAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 28637-1-AP

    WB analysis of A549 using 28637-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 28637-1-AP (ORAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for WB, ELISA
Cat No. 28637-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

CRACM1, FLJ14466, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, A375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28637-1-AP targets ORAI1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag29779

Product name: Recombinant human ORAI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-75 aa of BC015369

Sequence: MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ORAI calcium release-activated calcium modulator 1
别名 CRACM1, FLJ14466, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A
计算分子量 301 aa, 33 kDa
观测分子量32 kDa, 45-50 kDa
GenBank蛋白编号BC015369
基因名称 ORAI1
Gene ID (NCBI) 84876
RRIDAB_2881185
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96D31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

实验方案

Product Specific Protocols
WB protocol for ORAI1 antibody 28637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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