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验证数据展示

  • IF Staining of mouse eye using 25251-1-AP

    IF Staining of mouse eye using 25251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 25251-1-AP

    IF Staining of mouse eye using 25251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OPN5 Polyclonal antibody

OPN5 Polyclonal Antibody for IF-P, ELISA
Cat No. 25251-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, ELISA

别名

TMEM13, PGR12, opsin 5, NEUROPSIN, GPR136

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25251-1-AP targets OPN5 in IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 OPN5 fusion protein Ag19384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 opsin 5
别名 TMEM13, PGR12, opsin 5, NEUROPSIN, GPR136
计算分子量 354 aa, 40 kDa
GenBank蛋白编号BC126198
基因名称 OPN5
Gene ID (NCBI) 221391
RRIDAB_3669465
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6U736
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropsin (OPN5) is an opsin family member known as a photopigment responsive to wavelengths in the near-UV (λmax = 380 nm) (PMID: 31607531). In mammals, OPN5 is required to photoentrainment the local circadian oscillator in the murine retina and cornea (PMID: 21135214).

实验方案

Product Specific Protocols
IF protocol for OPN5 antibody 25251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols