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验证数据展示

  • WB analysis using 17445-1-AP

    WB analysis using 17445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17445-1-AP

    IP experiment of mouse brain using 17445-1-AP

    IP result of anti-Oligophrenin 1 (IP:17445-1-AP, 4ug; Detection:17445-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  • IHC staining of human colon using 17445-1-AP

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 17445-1-AP

    IHC staining of human colon using 17445-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

Oligophrenin 1 Polyclonal antibody

Oligophrenin 1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17445-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

MRX60, oligophrenin 1, OPHN1, OPN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17445-1-AP targets Oligophrenin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag11538

Product name: Recombinant human OPHN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-311 aa of BC059393

Sequence: MGHPPLEFSDCYLDSPDFRERLKCYEQELERTNKFIKDVIKDGNALISAMRNYSSAVQKFSQTLQSFQFDFIGDTLTDDEINIAESFKEFAELLNEVENERMMMVHNASDLLIKPLENFRKEQIGFTKERKKKFEKDGERFYSLLDRHLHLSSKKKESQLQEADLQVDKERHNFFESSLDYVYQIQEVQESKKFNIVEPVLAFLHSLFISNSLTVELTQDFLPYKQQLQLSLQNTRNHFSSTREEMEELKKRMKEAPQTCKLPGQPTIEGYLYTQEKCVWGHRGIHWVSISQELLPLVGCEFWAPLLFIDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oligophrenin 1
别名 MRX60, oligophrenin 1, OPHN1, OPN1
计算分子量 802 aa, 92 kDa
观测分子量 91 kDa
GenBank蛋白编号BC059393
基因名称 Oligophrenin 1
Gene ID (NCBI) 4983
RRIDAB_2158304
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60890
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

实验方案

Product Specific Protocols
WB protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IHC protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
IP protocol for Oligophrenin 1 antibody 17445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice

Authors - Margarita Brilkova
View Article