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验证数据展示

  • WB analysis of fetal human brain using 87815-1-RR

    WB analysis of fetal human brain using 87815-1-RR

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 87815-1-RR (OPCML antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 87815-1-RR

    WB analysis of rat brain using 87815-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 87815-1-RR (OPCML antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 87815-1-RR

    IF Staining of mouse brain using 87815-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OPCML antibody (87815-1-RR, Clone: 252030F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OPCML Recombinant monoclonal antibody

OPCML Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87815-1-RR

产品说明书

CloneNo. 252030F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

IgLON family member 1, IGLON1, OBCAM, Opioid-binding cell adhesion molecule, Opioid-binding protein/cell adhesion molecule

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, rat brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87815-1-RR targets OPCML in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6020

Product name: Recombinant human OPCM protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 28-322 aa of NM_001012393.5

Sequence: DATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 opioid binding protein/cell adhesion molecule-like
别名 IgLON family member 1, IGLON1, OBCAM, Opioid-binding cell adhesion molecule, Opioid-binding protein/cell adhesion molecule
计算分子量37 kDa
观测分子量40 kDa, 60 kDa
GenBank蛋白编号NM_001012393.5
基因名称 OPCML
Gene ID (NCBI) 4978
RRIDAB_3745766
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14982-2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPCML, also called opioid-binding cell adhesion molecule (OBCAM), belongs to the IgLON limbic system-associated membrane protein (LSAMP, OPCML/OBCAM, neurotrimin) family of IgG domain-containing glycosylphosphatidylinositol-anchored cell adhesion molecules. Originally isolated from the brain, OPCML is also expressed in the stomach, ovary, heart, placenta, testis, kidney, liver, pancreas, and colon. OPCML has been identified as a tumour suppressor, which inhibited tumor growth in some cancers, including ovarian cancer, lung cancer, and prostate cancer (PMID: 19176311; 28407749).

实验方案

Product Specific Protocols
IF protocol for OPCML antibody 87815-1-RRDownload protocol
WB protocol for OPCML antibody 87815-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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