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验证数据展示

  • WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

    WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

    IP experiment of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

    IP result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 21916-1-AP (same clone as 21916-1-PBS)

    IHC staining of human stomach cancer using 21916-1-AP (same clone as 21916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21916-1-AP (ONECUT2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ONECUT2 Polyclonal antibody, PBS Only

ONECUT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21916-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21916-1-PBS targets ONECUT2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16162

Product name: Recombinant human ONECUT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 226-332 aa of BC108925

Sequence: PLAATPLGNGLGGLHNAQQSLPNYGPPGHDKMLSPNFDAHHTAMLTRGEQHLSRGLGTPPAAMMSHLNGLHHPGHTQSHGPVLAPSRERPPSSSSGSQVATSGQLEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 one cut homeobox 2
别名
计算分子量 504 aa, 54 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC108925
基因名称 ONECUT2
Gene ID (NCBI) 9480
RRIDAB_2848180
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95948
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.

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