验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 2 publications below |
产品信息
25137-1-AP targets ONECUT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 |
CatNo: Ag18250 Product name: Recombinant human ONECUT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-292 aa of BC140830 Sequence: PYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTDKMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQVSNGSNSGQMEEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | one cut homeobox 1 |
| 别名 | Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1 |
| 计算分子量 | 465 aa, 51 kDa |
| 观测分子量 | 51-55 kDa |
| GenBank蛋白编号 | BC140830 |
| 基因名称 | ONECUT1 |
| Gene ID (NCBI) | 3175 |
| RRID | AB_2879918 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBC0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ONECUT1 antibody 25137-1-AP | Download protocol |
| WB protocol for ONECUT1 antibody 25137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6. | ||
Virol Sin Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters | ||
bioRxiv Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors | ||
Cells The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation | ||
Invest Ophthalmol Vis Sci Enhanced Transcriptional Activation in Developing Mouse Photoreceptors |
