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验证数据展示

  • WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

    WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

  • WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

    WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50571-3

    Cytometric bead array standard curve of MP50571-3

    Cytometric bead array standard curve of MP50571-3, OMA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66572-1-PBS. Detection antibody: 67449-1-PBS. Standard:Ag11201. Range: 3.125-100 ng/mL.

OMA1 Monoclonal antibody, PBS Only (Detector)

OMA1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67449-1-PBS

产品说明书

CloneNo. 1H4D12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67449-1-PBS targets OMA1 as part of a matched antibody pair:

MP50571-3: 66572-1-PBS capture and 67449-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 OMA1 fusion protein Ag11201 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
别名 Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-
计算分子量 524 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC012915
基因名称 OMA1
Gene ID (NCBI) 115209
RRIDAB_2882683
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96E52
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)