验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, PNGF treated mouse lung tissue |
| Positive IHC detected in | mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32685-1-AP targets OLR1/LOX1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2473 Product name: Recombinant Mouse OLR1/LOX1 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 60-363 aa of NM_138648.2 Sequence: RQVSDLLKQYQANLTQQDRILEGQMLAQQKAENTSQESKKELKGKIDTLTQKLNEKSKEQEELLQKNQNLQEALQRAANSSEESQRELKGKIDTITRKLDEKSKEQEELLQMIQNLQEALQRAANSSEESQRELKGKIDTLTLKLNEKSKEQEELLQKNQNLQEALQRAANFSGPCPQDWLWHKENCYLFHGPFSWEKNRQTCQSLGGQLLQINGADDLTFILQAISHTTSPFWIGLHRKKPGQPWLWENGTPLNFQFFKTRGVSLQLYSSGNCAYLQDGAVFAENCILIAFSICQKKTNHLQI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxidized low density lipoprotein (lectin-like) receptor 1 |
| 别名 | Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, Lectin-type oxidized LDL receptor 1, Lox1, LOX-1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 65-70 kDa, 37 kDa |
| GenBank蛋白编号 | NM_138648.2 |
| 基因名称 | Olr1 |
| Gene ID (NCBI) | 108078 |
| RRID | AB_3742705 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9EQ09 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). OLR1/LOX1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). Mouse OLR1/LOX1 is type II transmembrane protein containing 363 amino acids and has a cytoplasmic N-terminus and an extracellular C-terminus comprising the neck domain and the ligand-binding domain (CTLD) (PMID: 9588202). The molecular mass of mouse OLR1/LOX-1 at 65-70 kDa may be a fully glycosylated form, which is reduced to approximately 37 kDa after treatment with the deglycosylase PNGase.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for OLR1/LOX1 antibody 32685-1-AP | Download protocol |
| WB protocol for OLR1/LOX1 antibody 32685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
