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CoraLite® Plus 488-conjugated OLIG2 Monoclonal antibody

OLIG2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8C5

Cat No : CL488-66513

Print datasheet

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

验证数据展示

  • IF Staining of mouse brain using CL488-66513

    IF Staining of mouse brain using CL488-66513

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 OLIG2 antibody (CL488-66513, Clone: 1E8C5 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-66513

    IF Staining of mouse brain using CL488-66513

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 OLIG2 antibody (CL488-66513, Clone: 1E8C5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy OLIG2 Ready-To-Use IHC Kit. OLIG2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66513-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66513 targets OLIG2 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity Human, mouse, rat
Immunogen OLIG2 fusion protein Ag18838 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name oligodendrocyte lineage transcription factor 2
Synonyms BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 35-39 kDa
GenBank Accession NumberBC047511
Gene Symbol OLIG2
Gene ID (NCBI) 10215
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13516
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OLIG2 antibody CL488-66513Download protocol
Standard Protocols
Click here to view our Standard Protocols