验证数据展示
经过测试的应用
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10079-1-AP targets OLFM1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | OLFM1 fusion protein Ag0125 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | olfactomedin 1 |
| 别名 | |
| 计算分子量 | 20 kDa |
| GenBank蛋白编号 | BC000189 |
| 基因名称 | OLFM1 |
| Gene ID (NCBI) | 10439 |
| RRID | AB_2157220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99784 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Olfactomedin 1 (Olfm1), also known as noelin and pancortin, is a secreted glycoprotein belonging to a family of olfactomedin domain-containing proteins. The gene of Olfm1 is located on chromosome 9q34.3. Four variant transcripts (AMY, BMY, AMZ, and BMZ) are produced by alternative splicing. Olfm1 may be involved in the regulation of neural crest cell production, neuronal differentiation and ischemic neuronal death in adult. This antibody is specific for isoform 3 and isoform 4 (AMY) of Olfm1 with native molecular weights of 54 kDa and 16 kDa respectively. AMY may have an essential role in nerve tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for OLFM1 antibody 10079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |