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验证数据展示

  • WB analysis using 17676-1-AP

    WB analysis using 17676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17676-1-AP (OLAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

OLAH Polyclonal antibody

OLAH Polyclonal Antibody for WB, ELISA
Cat No. 17676-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Augmented in rheumatoid arthritis 1, AURA1, EC:3.1.2.14, Oleoyl-ACP hydrolase, S-acyl fatty acid synthase thioesterase, medium chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, MCF-10A cells, LNCap cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17676-1-AP targets OLAH in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 OLAH fusion protein Ag11694 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oleoyl-ACP hydrolase
别名 Augmented in rheumatoid arthritis 1, AURA1, EC:3.1.2.14, Oleoyl-ACP hydrolase, S-acyl fatty acid synthase thioesterase, medium chain
计算分子量 265 aa, 30 kDa
观测分子量36 kDa
GenBank蛋白编号BC050372
基因名称 OLAH
Gene ID (NCBI) 55301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NV23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OLAH protein, also known as oleoyl-ACP hydrolase, is an enzyme involved in fatty acid production. It has been found to play a role in various biological processes and diseases. For instance, placental OLAH levels are altered in fetal growth restriction and preeclampsia, suggesting its potential involvement in placental dysfunction. Additionally, OLAH has been linked to severe respiratory diseases, where it mediates life-threatening inflammation associated with viral infections. Its expression is also associated with fatal outcomes in certain viral infections. These findings highlight the importance of OLAH in both reproductive health and infectious diseases (PMID: 36139751, PMID: 39137778, PMID: 39178832). OLAH has two isoforms with molecular masses of 30 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for OLAH antibody 17676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols