验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | GTPBP9 fusion protein Ag9283 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Obg-like ATPase 1 |
| 别名 | OLA1, Obg-like ATPase 1, Obg like ATPase 1, GTP-binding protein 9, GTP binding protein 9 |
| 计算分子量 | 396aa,45 kDa; 278aa,31 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC013925 |
| 基因名称 | OLA1 |
| Gene ID (NCBI) | 29789 |
| RRID | AB_2157087 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NTK5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
| IHC protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
| IF protocol for GTPBP9 antibody 16371-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart | ||
Clin Exp Pharmacol Physiol OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.
| ||
medRxiv Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart | ||
PLoS One Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart | ||
J Cell Sci HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis
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