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Species Reactivity
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验证数据展示

  • WB analysis using 32301-1-AP

    WB analysis using 32301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32301-1-AP (OIT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

OIT3 Polyclonal antibody

OIT3 Polyclonal Antibody for WB, ELISA
Cat No. 32301-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Liver-specific zona pellucida domain-containing protein, LZP, Oncoprotein-induced transcript 3 protein, UNQ826/PRO1753

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32301-1-AP targets OIT3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 OIT3 fusion protein Ag36932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oncoprotein induced transcript 3
别名 Liver-specific zona pellucida domain-containing protein, LZP, Oncoprotein-induced transcript 3 protein, UNQ826/PRO1753
计算分子量60 kDa，545aa
观测分子量70 kDa
GenBank蛋白编号NM_152635.2
基因名称 OIT3
Gene ID (NCBI) 170392
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WWZ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Oncoprotein-induced transcript 3 protein (OIT3) is a liver-specific protein. It is involved in various biological processes, including lipid metabolism and ferroptosis regulation. OIT3 has been shown to mediate macrophage polarization and facilitate hepatocellular carcinoma (HCC) progression by regulating the arachidonic acid metabolism and reactive oxygen species (ROS) accumulation (PMID: 35353239). Additionally, OIT3 is implicated in the regulation of lipid metabolism, particularly in the context of triacylglycerol and very low-density lipoproteins (VLDLs) in the liver (PMID: 36132142).

实验方案

Product Specific Protocols
WB protocol for OIT3 antibody 32301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols