验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A549 cells, HEK-293 cells, L02 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11177-1-AP targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | OGFR fusion protein Ag1671 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | opioid growth factor receptor |
| 别名 | Zeta-type opioid receptor, Zeta type opioid receptor, Protein 7-60, Protein 7 60, Opioid growth factor receptor |
| 计算分子量 | 73 kDa |
| 观测分子量 | 90-110 kDa |
| GenBank蛋白编号 | BC014137 |
| 基因名称 | OGFR |
| Gene ID (NCBI) | 11054 |
| RRID | AB_2156772 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZT2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OGFR antibody 11177-1-AP | Download protocol |
| IHC protocol for OGFR antibody 11177-1-AP | Download protocol |
| IF protocol for OGFR antibody 11177-1-AP | Download protocol |
| IP protocol for OGFR antibody 11177-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.
| ||
Front Oncol Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study. | ||
Int Immunopharmacol Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor. | ||
Int Immunopharmacol Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma. | ||
Int Immunopharmacol Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. | ||
Int Immunopharmacol A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.
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