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验证数据展示

  • WB analysis using 21590-1-AP

    WB analysis using 21590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21590-1-AP (OGFOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21590-1-AP

    IHC staining of mouse brain using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OGFOD2 Polyclonal antibody

OGFOD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21590-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OGFOD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human testis tissue, human skeletal muscle tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21590-1-AP targets OGFOD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16164

Product name: Recombinant human OGFOD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC098293

Sequence: FTAPFCQALLEELEHFEQSDMPKGRPNTMNNYGVLLHELGLDEPLMTPLRERFLQPLMALLYPDCGGGRLDSHRAFVVKYAPGQDLELGCHYDNAELTLNVALGKVFTGGALYFGGLFQAPTALTEPLEVEHVVGQGVLHRGGQLHGARPLGTGERWNLVVWLRASAVRNSLCPMCCREPDLVDDEGFGDGFTREEPATVDVCALT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 2-oxoglutarate and iron-dependent oxygenase domain containing 2
别名 OGFOD2
计算分子量 350 aa, 39 kDa
观测分子量 32 kDa
GenBank蛋白编号BC098293
基因名称 OGFOD2
Gene ID (NCBI) 79676
RRIDAB_10734327
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6N063
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGFOD2 belongs to the OGFOD2 family. OGFOD2 has 4 isoforms with the molecular mass of 15, 20, 32 and 39 kDa.

实验方案

Product Specific Protocols
IHC protocol for OGFOD2 antibody 21590-1-APDownload protocol
WB protocol for OGFOD2 antibody 21590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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