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验证数据展示

  • WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82773-1-RR (same clone as 82773-1-PBS)

    WB analysis of HeLa using 82773-1-RR (same clone as 82773-1-PBS)

    WB result of OGDH antibody (82773-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OGDH transfected HeLa cells. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGDH antibody included
  • IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

OGDH Recombinant monoclonal antibody, PBS Only

OGDH Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 82773-1-PBS

产品说明书

CloneNo. 1C16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

1C16, 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, Alpha-ketoglutarate dehydrogenase, Alpha-KGDH-E1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.

产品信息

82773-1-PBS targets OGDH in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7368

Product name: Recombinant human OGDH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 674-1023 aa of BC004964

Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
别名 1C16, 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, Alpha-ketoglutarate dehydrogenase, Alpha-KGDH-E1
计算分子量 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC004964
基因名称 OGDH
Gene ID (NCBI) 4967
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ02218
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

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