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验证数据展示

  • WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGDH antibody included
  • IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGDH antibody included
  • IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

OGDH Monoclonal antibody, PBS Only

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66285-1-PBS

产品说明书

CloneNo. 1H7A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AKGDH, E1k, OGDC, OGDC E1, OGDH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.

产品信息

66285-1-PBS targets OGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 OGDH fusion protein Ag7484 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
别名 AKGDH, E1k, OGDC, OGDC E1, OGDH
计算分子量 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC004964
基因名称 OGDH
Gene ID (NCBI) 4967
RRIDAB_2881668
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02218
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.