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ODF2 Polyclonal antibody

ODF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12058-1-AP

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Synonyms

Cenexin, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2



经过测试的应用

Positive WB detected inmouse testis tissue, mouse lung tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

12058-1-AP targets ODF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ODF2 fusion protein Ag2693 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name outer dense fiber of sperm tails 2
Synonyms Cenexin, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2
Calculated molecular weight 763 aa, 89 kDa
Observed molecular weight 67-100 kDa
GenBank accession numberBC010629
Gene symbol ODF2
UniprotQ5BJF6
Gene ID (NCBI) 4957
RRIDAB_2156630
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

实验方案

Product Specific Protocols
WB protocol for ODF2 antibody 12058-1-APDownload protocol
IHC protocol for ODF2 antibody 12058-1-APDownload protocol
IF protocol for ODF2 antibody 12058-1-APDownload protocol
IP protocol for ODF2 antibody 12058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Centrosome anchoring regulates progenitor properties and cortical formation.

Authors - Wei Shao
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
mouseIF

Cell

Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility.

Authors - Jean-Ju Chung
humanIF

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu

Dev Cell

Rab11 endosomes contribute to mitotic spindle organization and orientation.

Authors - Heidi Hehnly
humanWB

J Exp Med

A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.

Authors - Beibei Zhang