验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
24736-1-AP targets ODF1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag20562 Product name: Recombinant human ODF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-250 aa of BC104457 Sequence: MAALSCLLDSVRRDIKKVDRELRQLRCIDEFSTRCLCDLYMHPYCCCDLHPYPYCLCYSKRSRSCGLCDLYPCCLCDYKLYCLRPSLRSLERKAIRAIEDEKRELAKLRRTTNRILASSCCSSNILGSVNVCGFEPDQVKVRVKDGKVCVSAERENRYDCLGSKKYSYMNICKEFSLPPCVDEKDVTYSYGLGSCVKIESPCYPCTSPCSPCSPCNPCNPCSPCNPCSPYDPCNPCYPCGSRFSCRKMIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | outer dense fiber of sperm tails 1 |
| 别名 | HSPB10, ODF, ODF1, ODF2, ODF27, ODFP, ODFPG, ODFPGA, ODFPGB, Outer dense fiber protein 1, RT7, SODF |
| 计算分子量 | 250 aa, 28 kDa |
| 观测分子量 | 28 kDa, 56 kDa |
| GenBank蛋白编号 | BC104457 |
| 基因名称 | ODF1 |
| Gene ID (NCBI) | 4956 |
| RRID | AB_2879697 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14990 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ODF1, also named as Outer dense fiber protein 1, is a 250 amino acid protein, which contains the C-terminal contains many C-X-P repeats. ODF1 is a component of the outer dense fibers (ODF) of spermatozoa. ODF27 that suggested the possibility of inter- actions between ODF27 itself and/or between ODF27 and other sperm tail proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ODF1 antibody 24736-1-AP | Download protocol |
| WB protocol for ODF1 antibody 24736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. | ||
Mol Genet Genomic Med Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects. | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice |
