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验证数据展示

  • WB analysis of RAW264.7 using 23454-1-AP

    WB analysis of RAW264.7 using 23454-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 23454-1-AP

    IHC staining of human prostate hyperplasia using 23454-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 23454-1-AP

    IHC staining of human prostate hyperplasia using 23454-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

ODC1 Polyclonal antibody

ODC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23454-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW264.7 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23454-1-AP targets ODC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ODC1 fusion protein Ag10927 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ornithine decarboxylase 1
别名 ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1
计算分子量 461 aa, 51 kDa
观测分子量 100 kDa
GenBank蛋白编号BC025296
基因名称 ODC1
Gene ID (NCBI) 4953
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11926
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

实验方案

Product Specific Protocols
WB protocol for ODC1 antibody 23454-1-APDownload protocol
IHC protocol for ODC1 antibody 23454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols