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验证数据展示

  • WB analysis using 13437-1-AP

    WB analysis using 13437-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 13437-1-AP (OCIAD2 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 13437-1-AP

    IHC staining of human stomach cancer using 13437-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 13437-1-AP

    IF Staining of U-251 using 13437-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using OCIAD2 antibody (13437-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human intrahepatic cholangiocarcinoma using 13437-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13437-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OCIAD2 Polyclonal antibody

OCIAD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13437-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Ovarian carcinoma immunoreactive antigen-like protein, OCIA domain-containing protein 2, OCIA domain containing 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13437-1-AP targets OCIAD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4243

Product name: Recombinant human OCIAD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC032808

Sequence: MASASARGNQDKDAHFPPPSKQSLLFCPKSKLHIHRAEISKIMRECQEESFWKRALPFSLVSMLVTQGLVYQGYLAANSRFGSLPKVARTASLPVRNAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OCIA domain containing 2
别名 Ovarian carcinoma immunoreactive antigen-like protein, OCIA domain-containing protein 2, OCIA domain containing 2
计算分子量 99 aa, 11 kDa
观测分子量17 kDa
GenBank蛋白编号BC032808
基因名称 OCIAD2
Gene ID (NCBI) 132299
RRIDAB_3085414
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ56VL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OCIAD2 antibody 13437-1-APDownload protocol
IHC protocol for OCIAD2 antibody 13437-1-APDownload protocol
IF protocol for OCIAD2 antibody 13437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols