验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
14809-1-AP targets OBFC2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6532 Product name: Recombinant human OBFC2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC001079 Sequence: MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oligonucleotide/oligosaccharide-binding fold containing 2B |
| 别名 | Sensor of single-strand DNA complex subunit B1, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Nucleic acid-binding protein 2, NABP2, LP3587 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 30-33 kDa |
| GenBank蛋白编号 | BC001079 |
| 基因名称 | OBFC2B |
| Gene ID (NCBI) | 79035 |
| RRID | AB_2251673 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQ15 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| IHC protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| IP protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| WB protocol for OBFC2B antibody 14809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res The Integrator complex controls the termination of transcription at diverse classes of gene targets.
| ||
Cell Death Dis Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability. | ||
J Inflamm Res Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma |
