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OAT Polyclonal antibody

OAT Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 17089-1-AP

Print datasheet

Synonyms

HOGA, OAT

验证数据展示

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17089-1-AP

    IF Staining of HepG2 using 17089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17089-1-AP (OAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17089-1-AP targets OAT in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human
Immunogen OAT fusion protein Ag9981 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ornithine aminotransferase (gyrate atrophy)
Synonyms HOGA, OAT
Calculated Molecular Weight 62 kDa
GenBank Accession NumberBC000964
Gene Symbol OAT
Gene ID (NCBI) 4942
RRIDAB_2878347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04181
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for OAT antibody 17089-1-APDownload protocol
IF protocol for OAT antibody 17089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols