验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
27143-1-AP targets Nogo receptor/NgR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26021 Product name: Recombinant human RTN4R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-473 aa of BC011787 Sequence: ALPTEALAPLRALQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCSLPQRLAGRDLKRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRPEGSEPPGFPTSGPRRRPGCSRKNRTRSHCRLGQAGSGGGGTGDSEGSGALPSLTCSLTPLGLALVLWTVLGPC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | reticulon 4 receptor |
| 别名 | RTN4R, NOGOR, Nogo-66 receptor, Nogo receptor, Nogo 66 receptor |
| 计算分子量 | 473 aa, 51 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC011787 |
| 基因名称 | RTN4R |
| Gene ID (NCBI) | 65078 |
| RRID | AB_2918118 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZR6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nogo receptor (NgR), also known as reticulon 4 receptor (RTN4R) or Nogo-66 Receptor, is a glycosylphosphatidylinositol (GPI)-linked, leucine-rich repeat (LRR) protein (PMID: 11201742). NgR is expressed predominantly by neurons in the brain. It is the receptor of Nogo-A (Nogo-66), oligodendrocyte myelin protein (OMgp) and myelin-associated glycoprotein (MAG), and mediates inhibition of axonal regeneration (PMID: 12694398). The apparent molecular wight of NgR detected by this antibody is 64 kDa, which is larger than the calculated molecular weight of NgR, probably due to glycosylation (PMID: 15751227; 12694398).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Nogo receptor/NgR1 antibody 27143-1-AP | Download protocol |
| WB protocol for Nogo receptor/NgR1 antibody 27143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis. | ||
Cell Transplant Human Umbilical Cord Mesenchymal Stem Cells Improve the Status of Hypoxic/Ischemic Cerebral Palsy Rats by Downregulating NogoA/NgR/Rho Pathway | ||
J Proteome Res Hypoxia Induces Extensive Protein and Proteolytic Remodeling of the Cell Surface in Pancreatic Adenocarcinoma (PDAC) Cell Lines |
