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Nlrp3 Polyclonal antibody

Nlrp3 Polyclonal Antibody for WB,IP,IF/ICC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB,IP,IF/ICC,ELISA

Conjugate

Unconjugated

Cat No : 30109-1-AP

Print datasheet

Synonyms

AGTAVPRL, AII/AVP, Cias1, FCAS, FCU, Mmig1, MWS, NALP3, Nlrp3, Pypaf1

验证数据展示

  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of RAW 264.7 using 30109-1-AP

    IP experiment of RAW 264.7 using 30109-1-AP

    IP result of anti-Nlrp3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:4000) with RAW 264.7 cells lysate 1200 ug.

  • IF Staining of HepG2 using 30109-1-AP

    IF Staining of HepG2 using 30109-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJ774A.1 cells, rat spleen tissue, RAW 264.7 cells, THP-1 cells, LPS treated RAW 264.7 cells
Positive IP detected inRAW 264.7 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30109-1-AP targets Nlrp3 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IP,IF/ICC,ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen Nlrp3 fusion protein Ag32515 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NLR family, pyrin domain containing 3
Synonyms AGTAVPRL, AII/AVP, Cias1, FCAS, FCU, Mmig1, MWS, NALP3, Nlrp3, Pypaf1
Calculated Molecular Weight118kd
Observed Molecular Weight108-120 kDa
GenBank Accession NumberNM_145827
Gene Symbol Nlrp3
Gene ID (NCBI) 216799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8R4B8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse Nlrp3.

实验方案

Product Specific Protocols
WB protocol for Nlrp3 antibody 30109-1-APDownload protocol
IF protocol for Nlrp3 antibody 30109-1-APDownload protocol
IP protocol for Nlrp3 antibody 30109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

JACC Basic Transl Sci

PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia

Authors - Meixin Zhang
View Article