验证数据展示
经过测试的应用
| Positive WB detected in | LPS treated RAW 264.7 cells, J774A.1 cells, rat spleen tissue, LPS and Brefeldin A treated NR8383 cells, RAW 264.7 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IF/ICC detected in | HepG2 cells, RAW 264.7 cells |
NLRP3 expression is relatively low in normal tissues(PMID: 33538177, 35676979, 25524927, 24166187)
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30109-1-AP targets NLRP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Western Blot applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IF/ICC, IP, CoIP, ELISA, Western Blot |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish, bovine |
| 免疫原 |
CatNo: Ag32515 Product name: Recombinant mouse NLRP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-153 aa of NM_145827 Sequence: MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NLR family, pyrin domain containing 3 |
| 别名 | AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3 |
| 计算分子量 | 118kd |
| 观测分子量 | 108-120 kDa |
| GenBank蛋白编号 | NM_145827 |
| 基因名称 | Nlrp3 |
| Gene ID (NCBI) | 216799 |
| RRID | AB_3086231 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8R4B8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NLRP3 antibody 30109-1-AP | Download protocol |
| IP protocol for NLRP3 antibody 30109-1-AP | Download protocol |
| WB protocol for NLRP3 antibody 30109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy PPARG activation by pioglitazone promotes mitophagy and inhibits the NLRP3 inflammasome to alleviate arthritic joint inflammation and bone damage. | ||
Nat Commun PDK4 drives abdominal aortic aneurysm by promoting smooth muscle cell metabolic reprogramming and NLRP3-mediated pyroptosis. | ||
Nat Commun Antioxidant lipid nanoparticles enhance mRNA stability for regeneration therapy and gene editing. | ||
ACS Nano Engineered Bacteria-Driven Biohybrid Microrobots Potentiate IL-2 Immunotherapy by Evoking Pyroptosis. | ||
J Adv Res Engineering a multifunctional nanoplatform with cascade catalytic and bidirectional immunomodulatory capacities for integrating sepsis therapy. | ||
J Adv Res PTPN22 as a therapeutic target in intervertebral disc degeneration: Modulating mitophagy and pyroptosis through the PI3K/AKT/mTOR axis |
