验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26983-1-AP targets Nir2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25357 Product name: Recombinant human PITPNM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1158-1243 aa of BC022230 Sequence: LSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol transfer protein, membrane-associated 1 |
| 别名 | DRES9, NIR 2, NIR2, PITPNM, PITPnm 1, PITPNM1, Rd9, RDGB, RDGB1, RDGBA, RDGBA1 |
| 计算分子量 | 135 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC022230 |
| 基因名称 | Nir2 |
| Gene ID (NCBI) | 9600 |
| RRID | AB_2880712 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00562 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for Nir2 antibody 26983-1-AP | Download protocol |
| WB protocol for Nir2 antibody 26983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
| ||
Biochim Biophys Acta Mol Cell Res The role of Nir2, a lipid-transfer protein, in regulating endothelial cell functions |
