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验证数据展示

  • WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 87471-1-RR (same clone as 87471-1-PBS)

    IF Staining of C6 using 87471-1-RR (same clone as 87471-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C6 cells using Nicastrin antibody (87471-1-RR, Clone: 252886F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • Affinity of 87471-1-RR

    Affinity of 87471-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87471-RR against Human Nicastrin were performed. The affinity constant is 1.23 nM.

Nicastrin Recombinant monoclonal antibody, PBS Only

Nicastrin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87471-1-PBS

产品说明书

CloneNo. 252886F8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0253, NCSTN, UNQ1874/PRO4317

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87471-1-PBS targets Nicastrin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5137

Product name: Recombinant human Nicastrin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 338-689 aa of BC047621

Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nicastrin
别名 KIAA0253, NCSTN, UNQ1874/PRO4317
计算分子量 709 aa, 78.4 kDa
观测分子量 110 kDa, 130 kDa
GenBank蛋白编号BC047621
基因名称 Nicastrin
Gene ID (NCBI) 23385
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92542
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease that cleaves many type I membrane proteins, including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytic processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

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