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验证数据展示

  • WB analysis of mouse lung using 30576-1-AP

    WB analysis of mouse lung using 30576-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 30576-1-AP

    IHC staining of mouse spleen using 30576-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 30576-1-AP

    IHC staining of mouse spleen using 30576-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30576-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Lipocalin-2/NGAL Polyclonal antibody

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30576-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Lcn2, Lipocalin-2, Siderocalin LCN2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue, mouse testis tissue
Positive IHC detected inmouse spleen tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30576-1-AP targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipocalin 2
别名 Lcn2, Lipocalin-2, Siderocalin LCN2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin
计算分子量23KD
观测分子量22-25 kDa
GenBank蛋白编号NM_008491.1
基因名称 NGAL
Gene ID (NCBI) 16819
RRIDAB_3669734
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11672
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Lipocalin-2/NGAL.

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/NGAL antibody 30576-1-APDownload protocol
IHC protocol for Lipocalin-2/NGAL antibody 30576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols