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验证数据展示

  • Neutralization experiment of NeutraKine® Noggin using 69010-1-Ig (same clone as 69010-1-PBS)

    Neutralization experiment of NeutraKine® Noggin using 69010-1-Ig (same clone as 69010-1-PBS)

    Recombinant human Noggin (Cat.NO. HZ-1118) inhibits BMP-4 induced alkaline phosphate production (using pNPP as chromogenic substrate for detection) by ATDC5 in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity of human Noggin (40 ng/mL HZ-1118) is neutralized by mouse anti-human Noggin monoclonal antibody 69010-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 5-20 μg/mL. The NeutraControl mouse anti-human Noggin monoclonal antibody 69510-1-Ig, generated from the same immunogen, could not neutralize human Noggin at the range of 0.0244-200 μg/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69010-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69010-1-Ig (same clone as 69010-1-PBS)

    ELISA experiment of Recombinant protein using 69010-1-Ig (same clone as 69010-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human Noggin (Cat.NO. HZ-1118) at 70 ng/well followed by blocking and adding serial diluted Noggin antibody 69010-1-Ig and 69510-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69010-1-PBS in a different storage buffer formulation.

NeutraKine® Noggin Monoclonal antibody, PBS Only

NeutraKine® Noggin Monoclonal Antibody for Neutralization, ELISA, Indirect ELISA
Cat No. 69010-1-PBS

产品说明书

CloneNo. 2A11C1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Neutralization, ELISA, Indirect ELISA

别名

NOG, Noggin, 2A11C1, SYM1, SYNS1

缓冲液配方:  Liquid
Lyophilized
Liquid
偶联物:  Unconjugated
规格价格库存

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产品信息

69010-1-PBS targets NeutraKine® Noggin in Neutralization, ELISA, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 Neutralization, ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human Noggin protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 noggin
别名 NOG, Noggin, 2A11C1, SYM1, SYNS1
基因名称 Noggin
Gene ID (NCBI) 9241
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDQ13253
储存缓冲液 PBS only, pH 7.3.
储存Store at -80°C.

背景介绍

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

This antibody can be used to neutralize the bioactivity of Noggin.

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