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验证数据展示

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50035-1

    Sandwich ELISA standard curve of MP50035-1

    Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

  • Sample test of MP50035-1

    Sample test of MP50035-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 5 days. For the PBMC supernatant cultured for 1 day, the mean IL-10 concentration was determined to be 431.4 pg/mL in unstimulated PBMC supernatant, 1,534.2 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean IL-10 concentration was determined to be 225.9 pg/mL in unstimulated PBMC supernatant, 634.8 pg/mL in PHA stimulated PBMC supernatant.

  • Affinity and Kinetic Characterization of 69018-1-Ig

    Affinity and Kinetic Characterization of 69018-1-Ig

    iolayer interferometry (BLl) kinetic assays of 69018-1-Ig against Human IL-10 were performed. The affinity constant is 0.377 nM.

NeutraKine® IL-10 Monoclonal antibody, PBS Only (Capture)

NeutraKine® IL-10 Monoclonal Antibody for IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 69018-1-PBS

产品说明书

CloneNo. 1E4F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

69018-1-PBS targets NeutraKine® IL-10 as part of a matched antibody pair:

MP50035-1: 69018-1-PBS capture and 69018-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 10
别名 IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10
基因名称 IL-10
Gene ID (NCBI) 3586
RRIDAB_2918838
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP22301
储存缓冲液 PBS only, pH 7.3.
储存Store at -80°C.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.

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