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验证数据展示

  • Neutralization experiment of NeutraKine®HGF using 69027-1-Ig (same clone as 69027-1-PBS)

    Neutralization experiment of NeutraKine®HGF using 69027-1-Ig (same clone as 69027-1-PBS)

    Recombinant human HGF (Cat.NO. HZ-1084) stimulates Saos-2 cells (human osteosarcoma cell) produce IL-11 in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human HGF (50 ng/mL HZ-1084) is neutralized by mouse anti-human HGF monoclonal antibody 69027-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 2-8μg/mL. This data was developed using the same antibody clone with 69027-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69027-1-Ig (same clone as 69027-1-PBS)

    ELISA experiment of Recombinant protein using 69027-1-Ig (same clone as 69027-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human HGF (Cat.NO. HZ-1084) at 70 ng/well followed by blocking and adding serial diluted Thrombopoietin antibody 69027-1-Ig and 69527-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69027-1-PBS in a different storage buffer formulation.

NeutraKine®HGF Monoclonal antibody, PBS Only

NeutraKine®HGF Monoclonal Antibody for Neutralization, ELISA
Cat No. 69027-1-PBS

产品说明书

CloneNo. 2D2B4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Neutralization, ELISA

别名

HGF, HGF Alpha, 2D2B4, Hepatocyte growth factor, Hepatocyte growth factor alpha chain

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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69027-1-Ig

Planning an IHC experiment? We recommend our IHCeasy HGF Ready-To-Use IHC Kit. HGF primary antibody included.

产品信息

69027-1-PBS targets NeutraKine®HGF in applications and shows reactivity with human samples.

经测试应用 Neutralization, ELISA Application Description
经测试反应性 human
免疫原 human HumankineHGF protein HZ-1084 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatocyte growth factor (hepapoietin A; scatter factor)
别名 HGF, HGF Alpha, 2D2B4, Hepatocyte growth factor, Hepatocyte growth factor alpha chain
基因名称 HGF
Gene ID (NCBI) 3082
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP14210
储存缓冲液 PBS Only, pH 7.4.
储存Store at -80°C.

背景介绍

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.

This antibody is used to neutralize the bioactivity of HGF.