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验证数据展示

  • Non-Neutralization experiment of HT-2 cells using 69512-1-Ig (same clone as 69512-1-PBS)

    Non-Neutralization experiment of HT-2 cells using 69512-1-Ig (same clone as 69512-1-PBS)

    Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69512-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-1080 using 69512-1-Ig (same clone as 69512-1-PBS)

    IF Staining of HT-1080 using 69512-1-Ig (same clone as 69512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using NeutraControl TGF beta 1 antibody (69512-1-Ig, Clone: 1D3D11 ) at dilution of 1:3963 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 69512-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69512-1-Ig (same clone as 69512-1-PBS)

    ELISA experiment of Recombinant protein using 69512-1-Ig (same clone as 69512-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69512-1-PBS in a different storage buffer formulation.

NeutraControl TGF beta 1 Monoclonal antibody, PBS Only

NeutraControl TGF beta 1 Monoclonal Antibody for IF/ICC, ELISA, Non-Neutralization
Cat No. 69512-1-PBS

产品说明书

CloneNo. 1D3D11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, ELISA, Non-Neutralization

别名

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 1D3D11, Latency-associated peptide

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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69512-1-Ig

Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

产品信息

69512-1-PBS targets TGF beta 1 in IF/ICC, ELISA, Non-Neutralization applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA, Non-Neutralization Application Description
经测试反应性 human
免疫原 human Humankine TGF beta 1 protein HZ-1011 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 1D3D11, Latency-associated peptide
计算分子量44 kDa
基因名称 TGFB1
Gene ID (NCBI) 7040
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP01137
储存缓冲液 PBS Only, pH 7.4.
储存Store at -80°C.

背景介绍

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody is a neutralizing control antibody for TGF beta 1, the immunogen is the same as Neutrakine 69012-1-Ig but could not neutralize human TGF beta 1.