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验证数据展示

  • Non-Neutralization experiment of Saos-2 using 69527-1-Ig (same clone as 69527-1-PBS)

    Non-Neutralization experiment of Saos-2 using 69527-1-Ig (same clone as 69527-1-PBS)

    Recombinant human HGF (Cat.NO. HZ-1084) stimulates Saos-2 cells (human osteosarcoma cell) produce IL-11 in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human HGF (50ng/mL HZ-1084) is neutralized by mouse anti-human HGF monoclonal antibody 69027-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 2-8μg/mL.The NeutraControl mouse anti-human HGF antibody 69527-1-Ig could recognize human HGF but could not neutralize human HGF acitivity in the range of 0.3125-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69527-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69527-1-Ig (same clone as 69527-1-PBS)

    ELISA experiment of Recombinant protein using 69527-1-Ig (same clone as 69527-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human HGF (Cat.NO. HZ-1084) at 70 ng/well followed by blocking and adding serial diluted Thrombopoietin antibody 69027-1-Ig and 69527-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69527-1-PBS in a different storage buffer formulation.

NeutraControl HGF Monoclonal antibody, PBS Only

NeutraControl HGF Monoclonal Antibody for ELISA, Non-Neutralization
Cat No. 69527-1-PBS

产品说明书

CloneNo. 2G4G8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

ELISA, Non-Neutralization

别名

HGF, HGF Alpha, 2G4G8, Hepatocyte growth factor, Hepatocyte growth factor alpha chain

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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69527-1-Ig

Planning an IHC experiment? We recommend our IHCeasy HGF Ready-To-Use IHC Kit. HGF primary antibody included.

产品信息

69527-1-PBS targets HGF in applications and shows reactivity with human samples.

经测试应用 ELISA, Non-Neutralization Application Description
经测试反应性 human
免疫原 human Humankine HGF protein HZ-1084 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatocyte growth factor (hepapoietin A; scatter factor)
别名 HGF, HGF Alpha, 2G4G8, Hepatocyte growth factor, Hepatocyte growth factor alpha chain
基因名称 HGF
Gene ID (NCBI) 3082
偶联类型 Unconjugated
形式 Lyophilized Powder
纯化方式Protein A purification
储存缓冲液 PBS Only, pH 7.4.
内毒素<0.1 EU/μg
复溶This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
稳定性与储存Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.

This antibody is a neutralizing control antibody for HGF, the immunogen is the same as Neutrakine 69027-1-Ig but could not neutralize human HGF.