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验证数据展示

  • WB analysis using 84429-5-RR (same clone as 84429-5-PBS)

    WB analysis using 84429-5-RR (same clone as 84429-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84429-5-RR (NRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 84429-5-RR (same clone as 84429-5-PBS)

    IF Staining of PC-3 using 84429-5-RR (same clone as 84429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Neuropilin 1 antibody (84429-5-RR, Clone: 241812G11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

Neuropilin 1/CD304 Recombinant monoclonal antibody, PBS Only

Neuropilin 1/CD304 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84429-5-PBS

产品说明书

CloneNo. 241812G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

neuropilin 1, NRP1, 241812G11, BDCA4, CD304

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84429-5-PBS targets Neuropilin 1/CD304 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg2331

Product name: Recombinant Human Neuropilin-1 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-644 aa of BC007533

Sequence: FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuropilin 1
别名 neuropilin 1, NRP1, 241812G11, BDCA4, CD304
观测分子量130-140 kDa
GenBank蛋白编号BC007533
基因名称 Neuropilin 1
Gene ID (NCBI) 8829
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO14786
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

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