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验证数据展示

  • WB analysis using 83620-5-RR

    WB analysis using 83620-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83620-5-RR

    FC experiment of HeLa using 83620-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

Neurofibromin Recombinant monoclonal antibody

Neurofibromin Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83620-5-RR

产品说明书

CloneNo. 240540F2
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

NF1, 240540F2, neurofibromin 1, NF 1, NF-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, PC-12 cell, NIH/3T3 cells, HEK-293T cells, HeLa cells
Positive FC (Intra) detected inHeLa cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy NF1 Ready-To-Use IHC Kit. NF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83620-5-RR targets Neurofibromin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag25799

Product name: Recombinant human Neurofibromin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-100 aa of M60915

Sequence: MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neurofibromin 1
别名 NF1, 240540F2, neurofibromin 1, NF 1, NF-1
计算分子量 319 kDa
观测分子量250-280 kDa
GenBank蛋白编号M60915
基因名称 Neurofibromin 1
Gene ID (NCBI) 4763
RRIDAB_3671233
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21359
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

实验方案

Product Specific Protocols
FC protocol for Neurofibromin antibody 83620-5-RRDownload protocol
WB protocol for Neurofibromin antibody 83620-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

JCO Precis Oncol

Combination of Selpercatinib and Trametinib Overcomes Resistance to RET Inhibitors in RET-Mutant Medullary Thyroid Carcinoma.

Authors - David Milewski
  • KO Validated
View Article
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