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验证数据展示

  • WB analysis using 83620-5-RR

    WB analysis using 83620-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83620-5-RR

    FC experiment of HeLa using 83620-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

Neurofibromin Recombinant monoclonal antibody

Neurofibromin Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83620-5-RR

产品说明书

CloneNo. 240540F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

NF1, 240540F2, neurofibromin 1, NF 1, NF-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, PC-12 cell, NIH/3T3 cells, HEK-293T cells, HeLa cells
Positive FC (Intra) detected inHeLa cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy NF1 Ready-To-Use IHC Kit. NF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83620-5-RR targets Neurofibromin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25799

Product name: Recombinant human Neurofibromin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-100 aa of M60915

Sequence: MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neurofibromin 1
别名 NF1, 240540F2, neurofibromin 1, NF 1, NF-1
计算分子量 319 kDa
观测分子量250-280 kDa
GenBank蛋白编号M60915
基因名称 Neurofibromin 1
Gene ID (NCBI) 4763
RRIDAB_3671233
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21359
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

实验方案

Product Specific Protocols
WB protocol for Neurofibromin antibody 83620-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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