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验证数据展示

  • IHC staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    IHC staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80293-5-RR (Nestin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Nestin antibody included
  • IHC staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    IHC staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80293-5-RR (Nestin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Nestin antibody included
  • IHC staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    IHC staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80293-5-RR (Nestin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Nestin antibody included
  • IF Staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    IF Staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Nestin antibody (80293-5-RR, Clone: 253261E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    IF Staining of rat brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Nestin antibody (80293-5-RR, Clone: 253261E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    IF Staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Nestin antibody (80293-5-RR, Clone: 253261E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    IF Staining of mouse brain using 80293-5-RR (same clone as 80293-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Nestin antibody (80293-5-RR, Clone: 253261E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80293-5-PBS in a different storage buffer formulation.

Nestin Recombinant monoclonal antibody, PBS Only

Nestin Uni-rAb® Recombinant Antibody for IHC, IF-P, Indirect ELISA
Cat No. 80293-5-PBS

产品说明书

CloneNo. 253261E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, Indirect ELISA

别名

NES

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

产品信息

80293-5-PBS targets Nestin in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nestin
别名 NES
计算分子量 177 kDa
GenBank蛋白编号NM_006617
基因名称 Nestin
Gene ID (NCBI) 10763
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48681
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

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