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验证数据展示

  • WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

    WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

    IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68420-1-Ig (same clone as 68420-1-PBS)

    IF Staining of HeLa using 68420-1-Ig (same clone as 68420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

Nesprin 3 Monoclonal antibody, PBS Only

Nesprin 3 Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA
Cat No. 68420-1-PBS

产品说明书

CloneNo. 1D1G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IF-P, Indirect ELISA

别名

1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68420-1-PBS targets Nesprin 3 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26012

Product name: Recombinant human C14orf49 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-300 aa of BC146604

Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 49
别名 1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3
观测分子量110 kDa
GenBank蛋白编号BC146604
基因名称 Nesprin 3
Gene ID (NCBI) 161176
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6ZMZ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

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