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Nephrin Polyclonal antibody

Nephrin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22912-1-AP

Print datasheet

Synonyms

CNF, Nephrin, NPHS1

验证数据展示

  • WB analysis using 22912-1-AP

    WB analysis using 22912-1-AP

    mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 22912-1-AP

    IHC staining of rat kidney using 22912-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 22912-1-AP

    IHC staining of rat kidney using 22912-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22912-1-AP targets Nephrin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Nephrin fusion protein Ag18994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nephrosis 1, congenital, Finnish type (nephrin)
Synonyms CNF, Nephrin, NPHS1
Calculated Molecular Weight 1241 aa, 135 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC156935
Gene Symbol NPHS1
Gene ID (NCBI) 4868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO60500
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

实验方案

Product Specific Protocols
WB protocol for Nephrin antibody 22912-1-APDownload protocol
IHC protocol for Nephrin antibody 22912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols