• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32147-1-AP

    WB analysis using 32147-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 32147-1-AP (Nectin-2/CD112 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 32147-1-AP

    IHC staining of mouse testis using 32147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32147-1-AP (Nectin-2/CD112 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Nectin-2/CD112 Polyclonal antibody

Nectin-2/CD112 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32147-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Mph, mHveB, HveB, Herpesvirus entry mediator B, Herpes virus entry mediator B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inC2C12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32147-1-AP targets Nectin-2/CD112 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poliovirus receptor-related 2
别名 Mph, mHveB, HveB, Herpesvirus entry mediator B, Herpes virus entry mediator B
计算分子量57 kDa
观测分子量65-70 kDa
GenBank蛋白编号NM_008990.3
基因名称 Nectin-2/CD112
Gene ID (NCBI) 19294
RRIDAB_3670207
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP32507-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

实验方案

Product Specific Protocols
WB protocol for Nectin-2/CD112 antibody 32147-1-APDownload protocol
IHC protocol for Nectin-2/CD112 antibody 32147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols