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验证数据展示

  • IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

    IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

    IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83883-5-RR (same clone as 83883-5-PBS)

    IHC staining of mouse colon using 83883-5-RR (same clone as 83883-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:9000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83883-5-RR

    Affinity and Kinetic Characterization of 83883-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

Mouse NCAM1/CD56 Recombinant monoclonal antibody, PBS Only

NCAM1/CD56 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83883-5-PBS

产品说明书

CloneNo. 240936B10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF/ICC, ELISA

别名

CD56, Ncam1, 240936B10, E NCAM, Ncam

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83883-5-PBS targets NCAM1/CD56 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0652

Product name: Recombinant Mouse NCAM-1/CD56 protein (His Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-711 aa of

Sequence: LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural cell adhesion molecule 1
别名 CD56, Ncam1, 240936B10, E NCAM, Ncam
计算分子量119 kDa
观测分子量120 kDa, 140 kDa, 180 kDa
基因名称 Ncam1
Gene ID (NCBI) 17967
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP13595-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).

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